uacc893 breast cancer cells Search Results


uacc893 atcc crl 1902 cell line  (ATCC)
92
ATCC uacc893 atcc crl 1902 cell line
Uacc893 Atcc Crl 1902 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
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cvd 1902 wcb  (ATCC)
94
ATCC cvd 1902 wcb
Cvd 1902 Wcb, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mcf10a cells  (ATCC)
99
ATCC mcf10a cells
BKCa channels regulate the transmembrane potential in breast cancer cells. (A) Breast cancer cells displayed higher levels of BKCa transcripts compared to benign mammary epithelial cells. Total RNA from <t>MCF10A</t> benign mammary epithelial cells and UACC893, MDA-MB-231 and SK-BR-3 beast cancer cells was assayed for BKCa transcript levels using quantitative RT-PCR. Statistically significant differences vs. the MCF10A cells. N=3, P≤0.05. (B) BKCa channel proteins (green) were detected in UACC893 (upper panels), MDA-MB-231 (middle panels) and SK-BR-3 (lower panels) cells using fluorescent immunocytochemistry. Nuclei were counterstained with propidium iodide (red) and the image series were acquired using Zeiss 510 laser confocal microscope. A series was reconstructed to generate three dimensional images rotated to present the lateral sides of the cells with apical surfaces facing atop of their respective image. MDA-MB-231 cells did not possess basal-apical differentiation and appear flat in the images. Experiments were repeated at least three times for each cell line. (C) IbTX differentially modulates transmembrane potential. UACC893 (upper panel), MDA-MB-231 (middle panel) and SK-BR-3 (lower panel) cells pre-loaded with 2 μmol/l DiBac4(3) in membrane potential buffer and supplemented with 10 nmol/l IbTX were observed using a Zeiss 510 laser confocal microscope for 20 min with images captured every minute. Average signal intensities from 5 to 7 regions of interest were calculated and plotted. Increased signal intensity signifies depolarization due to IbTX inhibition of BKCa channels by IbTX. Significant differences vs. start of experiment (1 min). N=3. IbTX, iberiotoxin.
Mcf10a Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/uacc893+breast+cancer+cells/MCF+10A/pmc04306270-15-4-9
Average 99 stars, based on 1 article reviews
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breast cancer cell lines  (ATCC)
99
ATCC breast cancer cell lines
BKCa channels regulate the transmembrane potential in breast cancer cells. (A) Breast cancer cells displayed higher levels of BKCa transcripts compared to benign mammary epithelial cells. Total RNA from <t>MCF10A</t> benign mammary epithelial cells and UACC893, MDA-MB-231 and SK-BR-3 beast cancer cells was assayed for BKCa transcript levels using quantitative RT-PCR. Statistically significant differences vs. the MCF10A cells. N=3, P≤0.05. (B) BKCa channel proteins (green) were detected in UACC893 (upper panels), MDA-MB-231 (middle panels) and SK-BR-3 (lower panels) cells using fluorescent immunocytochemistry. Nuclei were counterstained with propidium iodide (red) and the image series were acquired using Zeiss 510 laser confocal microscope. A series was reconstructed to generate three dimensional images rotated to present the lateral sides of the cells with apical surfaces facing atop of their respective image. MDA-MB-231 cells did not possess basal-apical differentiation and appear flat in the images. Experiments were repeated at least three times for each cell line. (C) IbTX differentially modulates transmembrane potential. UACC893 (upper panel), MDA-MB-231 (middle panel) and SK-BR-3 (lower panel) cells pre-loaded with 2 μmol/l DiBac4(3) in membrane potential buffer and supplemented with 10 nmol/l IbTX were observed using a Zeiss 510 laser confocal microscope for 20 min with images captured every minute. Average signal intensities from 5 to 7 regions of interest were calculated and plotted. Increased signal intensity signifies depolarization due to IbTX inhibition of BKCa channels by IbTX. Significant differences vs. start of experiment (1 min). N=3. IbTX, iberiotoxin.
Breast Cancer Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/uacc893+breast+cancer+cells/MCF7/pmc12120737-284-1-19
Average 99 stars, based on 1 article reviews
breast cancer cell lines - by Bioz Stars, 2026-10
99/100 stars
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rpmi 1640 medium  (ATCC)
99
ATCC rpmi 1640 medium
BKCa channels regulate the transmembrane potential in breast cancer cells. (A) Breast cancer cells displayed higher levels of BKCa transcripts compared to benign mammary epithelial cells. Total RNA from <t>MCF10A</t> benign mammary epithelial cells and UACC893, MDA-MB-231 and SK-BR-3 beast cancer cells was assayed for BKCa transcript levels using quantitative RT-PCR. Statistically significant differences vs. the MCF10A cells. N=3, P≤0.05. (B) BKCa channel proteins (green) were detected in UACC893 (upper panels), MDA-MB-231 (middle panels) and SK-BR-3 (lower panels) cells using fluorescent immunocytochemistry. Nuclei were counterstained with propidium iodide (red) and the image series were acquired using Zeiss 510 laser confocal microscope. A series was reconstructed to generate three dimensional images rotated to present the lateral sides of the cells with apical surfaces facing atop of their respective image. MDA-MB-231 cells did not possess basal-apical differentiation and appear flat in the images. Experiments were repeated at least three times for each cell line. (C) IbTX differentially modulates transmembrane potential. UACC893 (upper panel), MDA-MB-231 (middle panel) and SK-BR-3 (lower panel) cells pre-loaded with 2 μmol/l DiBac4(3) in membrane potential buffer and supplemented with 10 nmol/l IbTX were observed using a Zeiss 510 laser confocal microscope for 20 min with images captured every minute. Average signal intensities from 5 to 7 regions of interest were calculated and plotted. Increased signal intensity signifies depolarization due to IbTX inhibition of BKCa channels by IbTX. Significant differences vs. start of experiment (1 min). N=3. IbTX, iberiotoxin.
Rpmi 1640 Medium, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/uacc893+breast+cancer+cells/RPMI-1640+Medium/pm20214974-62-10-9
Average 99 stars, based on 1 article reviews
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london crl 2316 cell line  (ATCC)
95
ATCC london crl 2316 cell line
BKCa channels regulate the transmembrane potential in breast cancer cells. (A) Breast cancer cells displayed higher levels of BKCa transcripts compared to benign mammary epithelial cells. Total RNA from <t>MCF10A</t> benign mammary epithelial cells and UACC893, MDA-MB-231 and SK-BR-3 beast cancer cells was assayed for BKCa transcript levels using quantitative RT-PCR. Statistically significant differences vs. the MCF10A cells. N=3, P≤0.05. (B) BKCa channel proteins (green) were detected in UACC893 (upper panels), MDA-MB-231 (middle panels) and SK-BR-3 (lower panels) cells using fluorescent immunocytochemistry. Nuclei were counterstained with propidium iodide (red) and the image series were acquired using Zeiss 510 laser confocal microscope. A series was reconstructed to generate three dimensional images rotated to present the lateral sides of the cells with apical surfaces facing atop of their respective image. MDA-MB-231 cells did not possess basal-apical differentiation and appear flat in the images. Experiments were repeated at least three times for each cell line. (C) IbTX differentially modulates transmembrane potential. UACC893 (upper panel), MDA-MB-231 (middle panel) and SK-BR-3 (lower panel) cells pre-loaded with 2 μmol/l DiBac4(3) in membrane potential buffer and supplemented with 10 nmol/l IbTX were observed using a Zeiss 510 laser confocal microscope for 20 min with images captured every minute. Average signal intensities from 5 to 7 regions of interest were calculated and plotted. Increased signal intensity signifies depolarization due to IbTX inhibition of BKCa channels by IbTX. Significant differences vs. start of experiment (1 min). N=3. IbTX, iberiotoxin.
London Crl 2316 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/uacc893+breast+cancer+cells/HCC202/10__7554_slash_elife__58374-295-103-117
Average 95 stars, based on 1 article reviews
london crl 2316 cell line - by Bioz Stars, 2026-10
95/100 stars
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breast  (ATCC)
97
ATCC breast
BKCa channels regulate the transmembrane potential in breast cancer cells. (A) Breast cancer cells displayed higher levels of BKCa transcripts compared to benign mammary epithelial cells. Total RNA from <t>MCF10A</t> benign mammary epithelial cells and UACC893, MDA-MB-231 and SK-BR-3 beast cancer cells was assayed for BKCa transcript levels using quantitative RT-PCR. Statistically significant differences vs. the MCF10A cells. N=3, P≤0.05. (B) BKCa channel proteins (green) were detected in UACC893 (upper panels), MDA-MB-231 (middle panels) and SK-BR-3 (lower panels) cells using fluorescent immunocytochemistry. Nuclei were counterstained with propidium iodide (red) and the image series were acquired using Zeiss 510 laser confocal microscope. A series was reconstructed to generate three dimensional images rotated to present the lateral sides of the cells with apical surfaces facing atop of their respective image. MDA-MB-231 cells did not possess basal-apical differentiation and appear flat in the images. Experiments were repeated at least three times for each cell line. (C) IbTX differentially modulates transmembrane potential. UACC893 (upper panel), MDA-MB-231 (middle panel) and SK-BR-3 (lower panel) cells pre-loaded with 2 μmol/l DiBac4(3) in membrane potential buffer and supplemented with 10 nmol/l IbTX were observed using a Zeiss 510 laser confocal microscope for 20 min with images captured every minute. Average signal intensities from 5 to 7 regions of interest were calculated and plotted. Increased signal intensity signifies depolarization due to IbTX inhibition of BKCa channels by IbTX. Significant differences vs. start of experiment (1 min). N=3. IbTX, iberiotoxin.
Breast, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/uacc893+breast+cancer+cells/Hs+578T/pmc02994259-89-16-9
Average 97 stars, based on 1 article reviews
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bacteria strains jis l 1902 absorption method antibacterial activity value thymol pvb  (ATCC)
99
ATCC bacteria strains jis l 1902 absorption method antibacterial activity value thymol pvb
BKCa channels regulate the transmembrane potential in breast cancer cells. (A) Breast cancer cells displayed higher levels of BKCa transcripts compared to benign mammary epithelial cells. Total RNA from <t>MCF10A</t> benign mammary epithelial cells and UACC893, MDA-MB-231 and SK-BR-3 beast cancer cells was assayed for BKCa transcript levels using quantitative RT-PCR. Statistically significant differences vs. the MCF10A cells. N=3, P≤0.05. (B) BKCa channel proteins (green) were detected in UACC893 (upper panels), MDA-MB-231 (middle panels) and SK-BR-3 (lower panels) cells using fluorescent immunocytochemistry. Nuclei were counterstained with propidium iodide (red) and the image series were acquired using Zeiss 510 laser confocal microscope. A series was reconstructed to generate three dimensional images rotated to present the lateral sides of the cells with apical surfaces facing atop of their respective image. MDA-MB-231 cells did not possess basal-apical differentiation and appear flat in the images. Experiments were repeated at least three times for each cell line. (C) IbTX differentially modulates transmembrane potential. UACC893 (upper panel), MDA-MB-231 (middle panel) and SK-BR-3 (lower panel) cells pre-loaded with 2 μmol/l DiBac4(3) in membrane potential buffer and supplemented with 10 nmol/l IbTX were observed using a Zeiss 510 laser confocal microscope for 20 min with images captured every minute. Average signal intensities from 5 to 7 regions of interest were calculated and plotted. Increased signal intensity signifies depolarization due to IbTX inhibition of BKCa channels by IbTX. Significant differences vs. start of experiment (1 min). N=3. IbTX, iberiotoxin.
Bacteria Strains Jis L 1902 Absorption Method Antibacterial Activity Value Thymol Pvb, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
bacteria strains jis l 1902 absorption method antibacterial activity value thymol pvb - by Bioz Stars, 2026-10
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rhizobium radiobacter  (ATCC)
93
ATCC rhizobium radiobacter
BKCa channels regulate the transmembrane potential in breast cancer cells. (A) Breast cancer cells displayed higher levels of BKCa transcripts compared to benign mammary epithelial cells. Total RNA from <t>MCF10A</t> benign mammary epithelial cells and UACC893, MDA-MB-231 and SK-BR-3 beast cancer cells was assayed for BKCa transcript levels using quantitative RT-PCR. Statistically significant differences vs. the MCF10A cells. N=3, P≤0.05. (B) BKCa channel proteins (green) were detected in UACC893 (upper panels), MDA-MB-231 (middle panels) and SK-BR-3 (lower panels) cells using fluorescent immunocytochemistry. Nuclei were counterstained with propidium iodide (red) and the image series were acquired using Zeiss 510 laser confocal microscope. A series was reconstructed to generate three dimensional images rotated to present the lateral sides of the cells with apical surfaces facing atop of their respective image. MDA-MB-231 cells did not possess basal-apical differentiation and appear flat in the images. Experiments were repeated at least three times for each cell line. (C) IbTX differentially modulates transmembrane potential. UACC893 (upper panel), MDA-MB-231 (middle panel) and SK-BR-3 (lower panel) cells pre-loaded with 2 μmol/l DiBac4(3) in membrane potential buffer and supplemented with 10 nmol/l IbTX were observed using a Zeiss 510 laser confocal microscope for 20 min with images captured every minute. Average signal intensities from 5 to 7 regions of interest were calculated and plotted. Increased signal intensity signifies depolarization due to IbTX inhibition of BKCa channels by IbTX. Significant differences vs. start of experiment (1 min). N=3. IbTX, iberiotoxin.
Rhizobium Radiobacter, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/uacc893+breast+cancer+cells/Rhizobium+radiobacter/10__1016_slash_j__sajb__2019__10__005-52-5-16
Average 93 stars, based on 1 article reviews
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Image Search Results


BKCa channels regulate the transmembrane potential in breast cancer cells. (A) Breast cancer cells displayed higher levels of BKCa transcripts compared to benign mammary epithelial cells. Total RNA from MCF10A benign mammary epithelial cells and UACC893, MDA-MB-231 and SK-BR-3 beast cancer cells was assayed for BKCa transcript levels using quantitative RT-PCR. Statistically significant differences vs. the MCF10A cells. N=3, P≤0.05. (B) BKCa channel proteins (green) were detected in UACC893 (upper panels), MDA-MB-231 (middle panels) and SK-BR-3 (lower panels) cells using fluorescent immunocytochemistry. Nuclei were counterstained with propidium iodide (red) and the image series were acquired using Zeiss 510 laser confocal microscope. A series was reconstructed to generate three dimensional images rotated to present the lateral sides of the cells with apical surfaces facing atop of their respective image. MDA-MB-231 cells did not possess basal-apical differentiation and appear flat in the images. Experiments were repeated at least three times for each cell line. (C) IbTX differentially modulates transmembrane potential. UACC893 (upper panel), MDA-MB-231 (middle panel) and SK-BR-3 (lower panel) cells pre-loaded with 2 μmol/l DiBac4(3) in membrane potential buffer and supplemented with 10 nmol/l IbTX were observed using a Zeiss 510 laser confocal microscope for 20 min with images captured every minute. Average signal intensities from 5 to 7 regions of interest were calculated and plotted. Increased signal intensity signifies depolarization due to IbTX inhibition of BKCa channels by IbTX. Significant differences vs. start of experiment (1 min). N=3. IbTX, iberiotoxin.

Journal: Oncology Reports

Article Title: BKCa channel inhibitor modulates the tumorigenic ability of hormone-independent breast cancer cells via the Wnt pathway

doi: 10.3892/or.2014.3617

Figure Lengend Snippet: BKCa channels regulate the transmembrane potential in breast cancer cells. (A) Breast cancer cells displayed higher levels of BKCa transcripts compared to benign mammary epithelial cells. Total RNA from MCF10A benign mammary epithelial cells and UACC893, MDA-MB-231 and SK-BR-3 beast cancer cells was assayed for BKCa transcript levels using quantitative RT-PCR. Statistically significant differences vs. the MCF10A cells. N=3, P≤0.05. (B) BKCa channel proteins (green) were detected in UACC893 (upper panels), MDA-MB-231 (middle panels) and SK-BR-3 (lower panels) cells using fluorescent immunocytochemistry. Nuclei were counterstained with propidium iodide (red) and the image series were acquired using Zeiss 510 laser confocal microscope. A series was reconstructed to generate three dimensional images rotated to present the lateral sides of the cells with apical surfaces facing atop of their respective image. MDA-MB-231 cells did not possess basal-apical differentiation and appear flat in the images. Experiments were repeated at least three times for each cell line. (C) IbTX differentially modulates transmembrane potential. UACC893 (upper panel), MDA-MB-231 (middle panel) and SK-BR-3 (lower panel) cells pre-loaded with 2 μmol/l DiBac4(3) in membrane potential buffer and supplemented with 10 nmol/l IbTX were observed using a Zeiss 510 laser confocal microscope for 20 min with images captured every minute. Average signal intensities from 5 to 7 regions of interest were calculated and plotted. Increased signal intensity signifies depolarization due to IbTX inhibition of BKCa channels by IbTX. Significant differences vs. start of experiment (1 min). N=3. IbTX, iberiotoxin.

Article Snippet: UACC893, MDA-MB-231, SK-BR-3 and MCF10A cells were purchased from ATCC (Manassas, VA, USA).

Techniques: Quantitative RT-PCR, Immunocytochemistry, Microscopy, Membrane, Inhibition